RalA and RalB differentially regulate development of epithelial tight junctions

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RalA and RalB differentially regulate development of epithelial tight junctions

Tight junctions (TJs) are structures indispensable to epithelial cells and are responsible for regulation of paracellular diffusion and maintenance of cellular polarity. Although many interactions between TJ constituents have been identified, questions remain concerning how specific functions of TJs are established and regulated. Here we investigated the roles of Ral GTPases and their common ef...

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SnapShot: Epithelial Tight Junctions

Tight junctions form a morphological and functional border between the apical and basolateral cell surface domains that serves as a paracellular diffusion barrier, enabling epithelial cells to separate compartments of different composition. Tight junctions also contribute to the generation and maintenance of cell polarity and regulate signaling mechanisms that guide cell behavior, shape, and ge...

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RalA and RalB are members of the Ras-like GTP-binding protein family. To investigate if Ral GTPases play a role in development, temporal and spatial patterns of RalA and RalB gene expression were examined during embryogenesis. RalA and RalB had tissue-specific pattern of expression in developing organs. Both GTPases were expressed in endodermal derivatives in the digestive tract, kidney, mesone...

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Differential involvement of RalA and RalB in colorectal cancer

Mutationally activated K-Ras can utilize a multitude of downstream effector proteins to promote oncogenesis. While the Raf and phosphoinositol 3-kinase effector pathways are the best-studied and validated, recent studies have established the critical importance of Ral guanine nucleotide exchange factor (RalGEF) activation of the RalA and RalB small GTPases in cancer biology. Due to recent evide...

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The Ral family of small G proteins has been implicated in tumorigenesis, invasion, and metastasis. However, little emphasis has been placed on clarifying the individual roles of the two Ral proteins, RalA and RalB, in these processes in view of their high sequence homology. Here we analyze the separate contributions of RalA and RalB in regulating cell migration, a necessary component of the inv...

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ژورنال

عنوان ژورنال: Molecular Biology of the Cell

سال: 2011

ISSN: 1059-1524,1939-4586

DOI: 10.1091/mbc.e11-07-0657